Daily jobs:
before patching: Empty the vacuum bottle Check the wire on the pipette holder and the ground wire look OK (chlorided, not scratched) Check the air in the antivibration table and top up if necessary Check the bath temperature and adjust the temperature controller if necessary
at the end of the day: DO THE WASHING UP!!
Weekly jobs:
Chloride wires & clean pipette holder Polishing glass capillaries for patch pipettes Sylgard for coating (if you do single-channel work) Coating Petri dishes with Sylgard *(if you are working on axons) Check fine forceps and re-grind if necessary (ask Gordon to show you this one) Check Sylgard baths for axon incubation and *make new ones if necessary Fix any defects in setup that have been noticed during the week Check all solutions Top up the 3M KCl for storing the pH electrode |